Use of animals in research

Research using animals is tightly constrained by the law and researchers (grant holders and staff) share responsibility with veterinary and animal care staff for designing and conducting research in this area.

As well as fulfilling any legal responsibilities and following associated codes of practice, they are primarily responsible for applying the principles outlined below.

The principles follow the 3Rs – replacement, reduction and refinement – which provide a framework for humane and high quality research. The 3Rs are embedded in national and international legislation and regulations on the use of animals in scientific procedures.

Key principles

Researchers are expected to give appropriate consideration to the 3Rs in any research involving animals that has the potential to cause the animals harm. They must also explain in any research proposals (both for grants and in proposals and other information provided to ethics committees) how they have taken the 3Rs into account.

Researchers and associated veterinary and animal care staff should adopt a culture of care with regard to the animals and stay abreast of developments in good practice and advances in the 3Rs. They are expected to familiarise themselves with the NC3Rs website and organisation’s resources and publications.

Everyone using animals, whether for experimentation, testing, tissue samples, or breeding and maintaining stock colonies, is responsible for ensuring high levels of welfare and protection. They should seek advice on animal welfare from the NACWO, NVS and, where appropriate, others with relevant animal welfare expertise.

Ethics committees are responsible for reviewing animal use at a local level and addressing situations where there is a risk that the use of animals may be in conflict with their best welfare interests. The committees have a key role in ensuring high standards. We recommend that the research establishment’s ethics committee, whether the AWERB established under the ASPA or otherwise, should be central to implementation of this guidance.

Legislation and related guidance

Animals (Scientific Procedures) Act 1986

Consolidated version of the Animals (Scientific Procedures Act) 1986, amended 2012 (ASPA). The ASPA regulates procedures carried out on ‘protected animals’ (vertebrates and cephalopods) for scientific or educational purposes that may cause pain, suffering, distress or lasting harm. The Act also regulates the breeding and supply of certain species of animals for research purposes, and the methods used to kill protected animals. The Act is administered by the Home Office in England, Scotland and Wales, and the Department of Health) in Northern Ireland.

Guidance on the Operation of the Animals (Scientific Procedures) Act 1986

This Home Office document explains how the ASPA is administered and enforced, and provides detailed guidance to holders of establishment, project and personal licences, as well as licence applicants.

Code of practice for the housing and care of animals bred, supplied or used for scientific purposes

This Home Office code of practice sets out the standards of animal accommodation and care required under the ASPA and provides advice on how to comply with these requirements.

Key animal research regulations for countries around the world

The AAALAC International website, listing key animal research regulations from countries around the world.

General policies and guidance

Responsibility in the use of animals in bioscience research

This guidance sets out the expectations of the funding bodies for the responsible use of animals in research. It applies to the use of any vertebrate animals and cephalopods (live animals or animal products). Implementation of the principles in this guidance is a condition of receiving funds from the funding bodies, regardless of where in the world the research is conducted. The guidance is therefore useful for applicants and reviewers of research proposals.

Non-human primate accommodation, care and use

These guidelines apply to any research involving the use of non-human primates, conducted in the UK or overseas. Implementation of the principles in the guidelines is a condition of receiving funds for non-human primate research. Compliance with the guidelines is assessed as part of NC3Rs’ involvement in the peer review processes of the funding bodies (see below).

ARRIVE guidelines

These reporting guidelines are a checklist of information to include in publications describing animal research. They apply to any study involving live animals, including invertebrates, in any area of the biosciences, to ensure that studies are reported in enough detail to add to the knowledge base. This transparency enables readers and reviewers to scrutinise the research adequately, evaluate its methodological rigour, and reproduce the methods or findings. The funding bodies stipulate that publications resulting from funded research should adhere to the ARRIVE guidelines.

Experimental design assistant

The Experimental Design Assistant (EDA) is a free, secure online tool from the NC3Rs to help researchers design robust in vivo experiments, likely to yield reliable and reproducible results. The system uses computer-based logical reasoning to provide bespoke feedback on study designs, including dedicated support for randomisation, blinding and sample size calculation. The funding bodies recommend use of the EDA when preparing research proposals.

NC3Rs resource hub: Genetically altered mice

Information to help with the implementation of approaches to reduce and refine the use of genetically altered (GA) mice. This includes advice on good practice in colony management, and on dealing with challenging scenarios, when returning to GA mouse work following COVID-19 lockdown.

NC3Rs resource hub: Non-human primate welfare

Information and resources to improve the welfare of non-human primates used in research, including guidelines, websites and events.

NC3Rs peer review and advice service

Research proposals involving the use of non-human primates, cats, dogs and equines (and for some funding bodies, those involving pigs) are referred to the NC3Rs for 3Rs review. This helps to ensure that the animal welfare standards are genuinely high and exceed the legal minima, that overseas work is conducted to standards equivalent to those in the UK, and that experimental design is robust. NC3Rs’ advice is used during decisions on funding and when drafting the terms and conditions of grant awards. Further information about the NC3Rs review process, including tips for applicants, is given on the NC3Rs website.

Additional questions on the use of animals overseas

As opportunities for UK-based researchers to collaborate with researchers overseas have increased, so too has the importance of ensuring that animal welfare standards are appropriate and equivalent to those in the UK. Many funding bodies require applicants to complete additional questions on the use of rodents, rabbits, Xenopus and livestock species overseas (checklists) and to upload them with their application. A signed statement from both the UK and overseas principal investigators is also required in some cases.

Choosing contractors for animal research

A PDF presentation to advise applicants of the welfare standards expected by the funding bodies when contracting out animal studies. Applicants intending to place studies at contractors overseas are especially advised to view the presentation before choosing the contractor and submitting their application.

Standardised questions on the use of pigs (PDF, 40KB)

Additional questions for completion by BBSRC applicants proposing to use pigs.

Concordats

UAR guidance for researchers on communicating research (PDF, 2.3MB)

Concordat on openness on animal research